Extended Data Fig. 3: Cryo-EM data processing workflow for ROOLFirm. | Nature

Extended Data Fig. 3: Cryo-EM data processing workflow for ROOLFirm.

From: Cryo-EM structure of a natural RNA nanocage

Extended Data Fig. 3

a, Maximum-likelihood classification of cryo-EM data using cryoSPARC. b, Example of a micrograph with ROOLFirm particles. 6,390 micrographs were obtained and analysed. c, Fourier shell correlation curves as a function of resolution for the final octamer and tetramer maps shown in panels e and f. d, Euler angle distribution of particles contributing to the final reconstructions. e, f, Cryo-EM maps of the octamer (e) and tetramer (f), coloured according to local resolution.

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