Extended Data Fig. 4: Cryo-EM data processing workflow for the individual monomer of ROOLEfa. | Nature

Extended Data Fig. 4: Cryo-EM data processing workflow for the individual monomer of ROOLEfa.

From: Cryo-EM structure of a natural RNA nanocage

Extended Data Fig. 4

a, Maximum-likelihood classification of cryo-EM data using cryoSPARC. b, Example of a micrograph with ROOLEfa particles. 5,253 micrographs were obtained and analysed. c, Cryo-EM maps coloured according to local resolution. d, Fourier shell correlation curves as a function of resolution for the final maps shown in panels a and c. e, Euler angle distribution of particles contributing to the final reconstruction.

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