Extended Data Fig. 5: Structure determination of human TAS1R2-TAS1R3 heterodimer in glycerol-free condition and structural comparisons with reported sucralose-bound sweet taste receptor.
From: Structural and functional characterization of human sweet taste receptor
a, Size exclusion chromatogram profile of the apo TAS1R2-TAS1R3 heterodimer in the glycerol-free buffer condition. b, Representative cryo-EM micrograph (scale bars, 50 nm) (6,412 micrographs). c, Two-dimensional (2D) class averages of apo TAS1R2-TAS1R3 particles in the glycerol-free buffer condition, showing different particle view. d, The raw map for apo TAS1R2-TAS1R3 in the glycerol-free buffer condition. e, The raw map for apo TAS1R2-TAS1R3 in the glycerol-free buffer condition aligns well with that of apo TAS1R2-TAS1R3 map. f, The apo TAS1R2-TAS1R3 structure fits well into the two raw maps. g, Structural comparisons of reported full-length TAS1R2-TAS1R3 (PDB: 9NOR) with apo TAS1R2-TAS1R3 (left) (RMSD: 2.07 Å) and sucralose-bound TAS1R2-TAS1R3 (right) (RMSD: 0.99 Å), respectively.
