Extended Data Fig. 9: Structural comparison of VFTD and TMD in TAS1R2-TAS1R3 structure with other class C GPCRs and AlphaFold 3 predicted model of TAS1R2-TAS1R3 in complex with G protein.
From: Structural and functional characterization of human sweet taste receptor
a, Electrostatic potential mapped onto the cleft formed by LB1 and LB2 in the VFTDs of TAS1R2 and TAS1R3. b, The structural comparison of TMD between agonist-bound mGlu5 homodimer and sucralose bound TAS1R2-TAS1R3 heterodimer. c, ICL2 in TAS1R2 and TAS1R3 forms a similar short helix conformation with that in bitter taste receptors TAS2R14 and TAS2R46. d, Conformational changes of VFTDs from apo- to agonist-bound states in TAS1R2, GABAB1, mGlu5 and CaSR. e-f, Structural comparison of VFTDs between L-Gln bound medaka fish TAS1R2-TAS1R3 (PDB: 5X2M) and sucralose bound human TAS1R2-TAS1R3 (e), the ligands binding modes in TAS1R2 (f). g, The overall structure model of TAS1R2-TAS1R3 in complex with Gustducin (Gαgustducin β1γ2) predicted by AlphaFold3. h, AlphaFold3 predicted TAS1R2-Gαgustducin complex model is similar with that of TAS1R2TMD-miniGαs/gust (PDB: 9NOX).
