Extended Data Fig. 1: Construct design and protein property analysis of human TAS1R2-TAS1R3.
From: Structural and functional characterization of human sweet taste receptor
a, Schematic diagram of the modified constructs for human TAS1R2 and TAS1R3 used for structure determination. b, The representative SDS-PAGE and western blotting of the TAS1R2-TAS1R3 heterodimer sample (≥ 4 independent experiments). The presence of TAS1R2 and TAS1R3 was confirmed by western blotting with anti-TAS1R2 and anti-TAS1R3 antibody, respectively. c, Analytical size-exclusion chromatograph (aSEC) analysis of the TAS1R2-TAS1R3 heterodimer sample, using UV280 absorbance and fluorescence detection. Left vertical axis: UV280 absorbance values (protein elution profile); Right vertical axis: normalized fluorescence values. Fluorescence detection parameters: TAS1R2-mScarlet3: Excitation 569 nm/ Emission 592 nm; for TAS1R3-mNeonGreen: Excitation 506 nm/ Emission 517 nm. d, Dose-response curves of glycerol on WT human sweet taste receptor measured in calcium mobilization assay. Data are mean ± s.e.m. of three independent experiments performed in triplicate.
