Extended Data Fig. 3: Structure determination of human TAS1R2-TAS1R3 heterodimer in sucralose bound state.
From: Structural and functional characterization of human sweet taste receptor
a, Size exclusion chromatogram profile of the TAS1R2-TAS1R3 in complex with sucralose. b, Representative cryo-EM micrograph (scale bars, 50 nm) (120,009 micrographs). c, Two-dimensional (2D) class averages of TAS1R2-TAS1R3 in complex with sucralose, showing different particle views. d, Data processing pipeline for the sucralose bound TAS1R2-TAS1R3 complex, including 2D classification and 3D reconstruction workflow and final 3D density map colored according to local resolution (Å). e, Fourier shell correlation (FSC) curves for the final 3D density maps of the TAS1R2-TAS1R3 in complex with sucralose. Euler Angle Distributions of the particles used for the final 3D reconstruction are also shown.
