Extended Data Fig. 7: Univariate differential expression analysis of whole blood transcripts in Groups 7–9.

For this analysis, Groups 8 and 9 were pooled, whereas Group 7 was examined separately. a,b, Volcano plots, as described in Extended Data Fig. 6, depicting the statistical significance and fold change (FC) in gene expression in differentially expressed genes measured at baseline (day 0) in Group 7 (a) and Groups 8 + 9 (b). The Wald test default method in Sleuth was used to assess whether the estimated coefficients (i.e., FCs) for each DEG was significantly different from zero. All P values are two-sided. NS, not significant. c,d, Dot plots showing overrepresented pathways by genes that are upregulated at week 4 in Group 7 (c) and Groups 8 + 9 (d), as annotated in the Reactome database.