Extended Data Fig. 6: Our informatics data processing workflow.
a, Overview of our informatics data processing workflow. b, Microphotographs of representative PHAL and CTB coinjections in the ACAv. Left panel shows a composed view of two channels where the injection site is overexposed. Middle and right panels show the same PHAL and CTB injection sites following their rescan with lower exposure parameters. Note the reduction of the injection site size following the rescan. All our injection sites are rescanned to attain an improved assessment of the injection site spread. c, Mapping of raw images onto their corresponding level of the ARA11 atlas using our registration pipeline. d, Accuracy of the registration pipeline demonstrated by precise mapping of CTB labelling in small nuclei, such as CLA, and laminar structures, such as cortex and SUB. e, Quantitative comparison showing that the semi-automatic registration resulted in fewer errors compared to automatic registration. f, Global connectivity matrix from representative cases with injections in the DPs, DPd, ILA, PL, ACAv, and ACAd. Despite individual variability, injections in the same MPF areas exhibit consistent connectivity patterns, while different MPF areas display distinct patterns. This consistency highlights the reliability and accuracy of our injections, supporting the validity of the experimental approach. Refer to the Supplementary Methods sections Statistical Model of Brain Connectivity and Quantifying Global MPF Brain Connectivity for further details on the quantification and construction of the connectivity matrix.
