Extended Data Fig. 2: Gene expression defining regional and laminar specificity of DP.

a, RNAscope images of marker genes, Cux2, Etv1, and Tle4, reveal distinct laminar expression in DPs and DPd. b-g, Allen Brain Atlas in situ hybridization data (mouse.brain-map.org/)73 across rostral (top) and caudal (bottom) DP levels. Cacna1h and Plxnd1 (L5 markers; Plxnd1 also in L2) extend into DPs; Tle4 (L6 CT marker) is restricted to DPd. Etv1 and Fezf2 (L5 CPT/IT markers) are expressed in both DPs and DPd. Cux2 (L2/3 IT marker) does not extend into DP, marking its dorsal boundary; Lrmp2 (TTd marker) defines the ventral border. Htr2c and Pamr1 are expressed in DPd and deep DPs, while Fxyd6 is enriched in DPd.