Extended Data Fig. 6: The spatial distribution of different segments of LSU pre-rRNAs in H9, H9-differentiated D30 arcuate neurons and SH-SY5Y cells.
From: Pre-rRNA spatial distribution and functional organization of the nucleolus
a. ITS2 localization in the PDFC–GC region remains unchanged across different cell types. Left, schematic of the ITS2 probe position. Middle, representative single-slice and averaged SIM images of ITS2 (magenta) and FBL (green). Right, the corresponding relative line intensity plots of ITS2 and FBL. n = 50, 22, 40 DFC units in 15 (H9), 10 (D30 arcuate neuron), 8 (SH-SY5Y) cells, respectively. b. 3′ ETS localization in the DFC–PDFC regions remains consistent across different cell types. Left, schematic of the 3′ ETS probe position. Middle, representative single-slice and averaged SIM images of 3′ ETS (magenta) and FBL (green). Right, the corresponding relative line intensity plots of 3′ ETS and FBL. n = 52, 10, 50 DFC units in 14 (H9), 7 (D30 arcuate neuron), 13 (SH-SY5Y) cells, respectively.
