Extended Data Fig. 8: Blocking SSU pre-rRNA processing alters pre-rRNA spatial localization and FC–DFC unit organization.
From: Pre-rRNA spatial distribution and functional organization of the nucleolus
a. Left top, ASOs incorporating phosphorothioate and 2’-MOE modifications. Representative SIM images showing the localization patterns of 5′ ETS-3 (magenta) in ASOs-treated HeLa cells. FCs are labelled by RPA194 (blue) and DFC by DKC1 (green). b. Representative SIM images showing normal and enlarged FC–DFC units. FCs are labelled by RPA194 (magenta), DFC by DKC1 (green), and GC by B23 (blue). Insets show magnified views of individual FC/DFC units. c. Representative SIM images showing fragmented FC–DFC units in ASO-Site A0 and ASO-Site 1 treated cells. d. Left, schematic illustration of three types of FC–DFC units: normal, enlarged and fragmented. Right, proportion of normal, enlarged, and fragmented FC–DFC units under each treatment condition. Data were obtained from n = 51, 27, 48 cells for ASO-Scr., ASO-Site A0, ASO-Site 1, respectively.
