Extended Data Fig. 4: Analysis of the altered relative FC/DFC interface in morphologically different nucleoli from different cells.
From: Pre-rRNA spatial distribution and functional organization of the nucleolus
a. Number of FC–DFC units during H9 cell differentiation to arcuate neurons. Data are mean ± s.e.m. n = 31, 31, 31, 47, 33, 31, 31, 32 cells across the sequential time points. b. Average FC volume in H9, D30 arcuate neurons, HeLa, and SH-SY5Y cells. Data are mean ± s.e.m. n = 45, 45, 48 and 45 cells, respectively. Statistical analysis was performed using two-tailed Student’s t-test. c. Visualization of the multi-layered nucleolar structure in D30 arcuated neurons. SIM images show the spatial organization of FBL (DFC, green) and B23 (GC, white), illustrating compartmentalized nucleolar subdomains. d. Schematic and theoretical model for calculating the Relative FC/DFC interface. Left, regular and irregular FC–DFC unit structures are illustrated, with the FC/DFC contact area normalized to FC volume to define a relative interface. Middle, equations detail the derivation of the Relative FC/DFC interface for spherical or irregular FCs based on radius or effective radius (r or rreff). Right, theoretical relationship showing the inverse correlation between Relative FC/DFC interface and FC radius.
