Fig. 3: p53 Hyperactivity suppresses chromatin noise and mTEC plasticity.
From: Thymic epithelial cells amplify epigenetic noise to promote immune tolerance
a, UMAP visualization of merged scATAC-seq profiles of mTECs from the indicated genotypes (colours) generated from Multiome datasets. b, Cluster annotations overlaid on the UMAP from a. c, Paired violin plots comparing distributions of the fraction of scATAC-seq fragments within scATAC-seq peaks (WIP) from the indicated genotypes across annotated clusters of mTEC maturation. P-values were calculated by one-sided Mann–Whitney U tests. d, Differences in the number (left) or sum of mRNA (right) of expressed αTSGs between neighbouring p53-cHyper and p53-WT mTECs overlaid on the UMAP from a. e, Scatter plot comparing the differential expression of genes by bulk RNA-seq resulting from mTEC maturation (horizontal axis) or p53 hyperactivity in mature mTECs (vertical axis) (n = 3 biological replicates). Fold-change densities are shown on the top and right margins. Statistically significant (Benjamini–Hochberg FDR ≤ 0.05) differential expression between p53-cHyper and p53-WT mTECs is highlighted in blue for all genes and red for αTSGs. f, UMAP of merged scRNA-seq profiles of p53-WT and p53-cHyper mTECs with cluster annotations overlaid. g, Differential density of genotypes (p53-cHyper versus p53-WT) across the mimetic mTEC clusters defined in f. h, Fraction of the indicated mimetic mTEC subtypes represented by each genotype. i, Comparison of the numbers of the indicated mimetic mTEC compartments between p53-WT and p53-cHyper sex-matched littermates (n = 6). P-values for two-sided paired ratio tests are shown.
