Extended Data Fig. 6: Arginine-induced allostery is required to disrupt the GATOR2-CASTOR1 interaction.
From: Structural basis for the dynamic regulation of mTORC1 by amino acids
(a) Disruption of the CASTOR1 loop β6-α3 and α3 helix stabilizes the GATOR2-CASTOR1 interaction. Anti-HA immunoprecipitates (IPs) were prepared from HEK293T cells transiently expressing the indicated cDNAs and deprived of all amino acids for 60 min and were analyzed by immunoblotting for the indicated proteins. (b) The CASTOR1 loop β6-α3 and α3 helix are required for arginine-induced disruption of the GATOR2-CASTOR1 complex. Anti-FLAG immunoprecipitates were prepared from HEK293T cells transiently expressing the indicated cDNAs and deprived of all amino acids for 60 min. Where indicated, arginine (400 μM) was added directly to the immunoprecipitates, which, after re-washing, were analyzed as in (a). Data in (a) and (b) are representative of two independent experiments. For gel source data, see Supplementary Fig. 1.
