Extended Data Fig. 9: Quantification of the WDR24 β-propeller dynamics.
From: Structural basis for the dynamic regulation of mTORC1 by amino acids
(a) Flowchart depicting the workflow to quantitatively determine the WDR24 β-propeller occupancy between the apo GATOR2 state and the inhibitor-bound sc-GATOR2-Sestrin2 and sc-GATOR2-CASTOR1 complexes. The solvent masks applied for particle subset selection in 3D classification are shown in green and applied focus masks are shown in blue. The shown particle subset maps are representative examples of the respective classification. (b) Epitope tagging of the WDR24 N-terminal β-propeller does not disrupt interactions between GATOR2-GATOR1-KICSTOR. Anti-MYC immunoprecipitates from WDR24-deficient HEK293T cells transiently expressing the indicated cDNAs were prepared and analyzed by immunoblotting for the indicated proteins. Data in (b) are representative of two independent experiments. For gel source data, see Supplementary Fig. 1.
