Fig. 1: Study design and characterization of droplet-based snRNA-seq in human PFC.
From: Single-cell transcriptomic and genomic changes in the ageing human brain
a, Overall study design. Human PFC was analysed by three single-cell genomic techniques in parallel. b, Dimensional reduction and clustering of all snRNA-seq nuclei after filtration yielded several clusters for each cell type (Ast, astrocyte; CC, cortico-cortico; Endo, endothelial; L, layer; Oli, oligodendrocyte; UMAP, uniform manifold approximation and projection). c, Percentage of nuclei in each cluster of our data that correspond to the annotated reference cluster. d, Gene-expression profiles for each subcluster within a cell type correspond most closely to the cells of the same lineage based on Pearsonâs correlation coefficient. The bar plot above the heat map shows the number of genes expressed in each cluster.
