Extended Data Fig. 4: Mutations of the CB1 receptor and the effects on agonist-induced activity as assessed by the forskolin-stimulated accumulation of cAMP.
From: Crystal structures of agonist-bound human cannabinoid receptor CB1
a, Primers used to generate mutations in 3×HA–CB1 and validation of cell-surface expression of wild-type and mutant CB1 in CHO-K1 cell lines quantitative flow cytometry. b, Dose response studies of agonist (AM11542, AM841 and CP55,940) activity for each mutant compared to wild type (in blue filled circles) from Fig. 3c. c, Assessment of the effect of the individual point mutations that were made to stabilize the receptor, in absence of the flavodoxin insert, on receptor activity. All experiments were repeated at least three times, and error bars denote s.e.m. of duplicate measurements (parameters are in Extended Data Table 2).
