Fig. 2: FASN translocates to peroxisomes, drives mmBCFA synthesis and regulates thermogenesis in BAT.
From: Peroxisomal metabolism of branched fatty acids regulates energy homeostasis
a, mRNA levels of mmBCFA β-oxidation and synthesis genes in BAT of WT mice maintained at thermoneutrality (30 °C) or 4 °C for 7 days. n = 4. b, Immunofluorescence analysis and quantitative analysis of FASN peroxisomal localization in BAT of WT mice maintained at room temperature (RT) or after cold treatment for 2 h. n = 3. c, Western blot analysis following subcellular fractionation in differentiated BAT SVF cells after norepinephrine (0.2 μM) treatment for 2 h. d, Western blot analysis of FASN in BAT and iWAT of FASN-BKO mice. n = 3. e, Mass spectrometry analysis of a StCFA C16:0 and an mmBCFA iso-C17:0 in BAT of FASN-BKO and control mice housed at 22 °C or 4 °C for 6 h. n = 4. f, Core body temperature of control (n = 13) and FASN-BKO (n = 12) mice after cold exposure (4 °C). g, Energy expenditure of control (n = 4) and FASN-BKO (n = 6) mice after CL316,243 treatment. Data in a,b (right),c–g are from biologically independent samples. Images in b (left) and c are representative of two separate experiments. Data are presented as mean ± s.e.m. Statistical analyses were performed using two-sided unpaired Student’s t-test (a,b right), one-way ANOVA (e) or two-way ANOVA (f–g), followed by Fisher’s least-significant difference post hoc test (e–g). Scale bars, 10 μm.
