Extended Data Fig. 3: Brown adipocyte-specific FASN knockout reduces mmBCFA levels and increases diet-induced obesity.
From: Peroxisomal metabolism of branched fatty acids regulates energy homeostasis
a, Western blot analysis of FASN in 4-hydroxytamoxifen (4-OHT)-treated FASNLox/Lox brown adipocytes transduced with retrovirus expressing GFP or Cre-ERT2. N = 3. b, Microscopy images of FASN-iKO and control BAT adipocytes prior to and after 4-OHT treatment. In the bottom panel, the cells were stained with Oil Red O. Scale bar, 75 mm (upper panel), 300 mm (lower panel). c, Mass spectrometry analysis of mmBCFA in control and FASN-iKO brown adipocytes. N = 3. d, Mass spectrometry analysis of various straight chain fatty acids in control and FASN-iKO brown adipocytes. N = 3. e, Body weight of HFD-fed FASN-BKO and control female mice. N = 7. f, Regression analysis of energy expenditure in HFD-fed mice. N = 7. g, Cumulative energy intake in HFD-fed FASN-BKO and control female mice. N = 4. h, Locomotor activity in FASN-BKO and control female mice. N = 4. Data are reported as mean ± SEM. Data in a and c-h are from biologically-independent samples. Images in b are representative of two separate experiments. Two-sided unpaired Student’s t test in c and d; two-way ANOVA with Fisher’s LSD in e; and two-way ANCOVA with Tukey’s test in f.
