Extended Data Fig. 4: Global knockout of ACOX2 impairs thermogenesis and exacerbates diet-induced obesity and metabolic dysfunction.

a, ACOX2 gene expression in adipose tissue depots of ACOX2^−/− and WT mice. N = 3. b, Western blot analysis of ACOX2 in BAT of ACOX2^−/− and WT mice. N = 2. c, Core body temperature of ACOX2^−/− and WT male mice after cold exposure (4 °C). N = 10. d, Gene expression analysis of proteins involved in UCP1-dependent and -independent pathways of thermogenesis in BAT of ACOX2^−/− and WT mice. N = 5. e, Gene expression analysis of WT mice fed normal chow diet or HFD. N = 3. f, Body weight of HFD-fed ACOX2^−/− and WT female mice. N = 7. g, Body weight of ACOX2^−/− and WT male mice fed with HFD. N = 9. h, Body composition analysis of ACOX2^−/− and WT male mice fed HFD. N = 9. i, Food intake of ACOX2^−/− and WT mice. N = 5. j, Locomotor activity of ACOX2^−/− and WT mice. N = 5. k, Tissue weights of adipose depots of HFD-fed ACOX2^−/− and WT male mice. N = 9. l, Gross images of BAT and iWAT from HFD-fed ACOX2^−/− and WT male mice. m, Histologic analysis of H&E-stained adipose tissue sections from HFD-fed ACOX2^−/− and WT male mice. Scale bar, 75 mm. n, GTT analysis in HFD-fed ACOX2^−/− and WT female mice. N = 7. o, ITT analysis in HFD-fed WT and ACOX2^−/− female mice. N = 7. Data are reported as the mean ± SEM. Data in a-k and n-o are from biologically independent samples. Images in l and m are representative of 3 mice/group. Two-sided unpaired Student’s t test in a, d, e, and h-k; two-way ANOVA followed by Fisher’s LSD test in c, f, g and n-o.

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