Extended Data Fig. 7: RNA-seq data for club neurons and calcium signals in club axons.

a, Expression levels of receptor genes in club neurons. b, Left, confocal image of club axons in the neuromere of the left front leg. Black box, imaging region; green, GFP; gray, neuropil stain (nc82); A, anterior; L, left. Right, mean tdTomato signal of a representative trial. Expression was consistent across animals (N = 6). c, Example of calcium imaging of club axons during behavior on the treadmill. d, Cross-correlation between predicted and measured calcium signals per trial at a time lag of zero (N = 6 flies, n = 48 trials in total). Black line, median; black dot, trial shown in c. e, Predicted and measured calcium signals aligned to the transitions into and out of movement (N = 6 flies). Movement includes walking and grooming. Signals are baseline subtracted (mean from −0.5 to 0 s). Thin lines, animal means; thick lines, mean of means; shadings, s.e.m. f, Example of calcium imaging of club axons without the treadmill. g, Same as d but for club axons imaged without the treadmill (N = 5 flies, n = 41 trials in total). h, Same as e but for club axons imaged without the treadmill (N = 5 flies). i, Example of calcium imaging of club axons in which the treadmill was lowered out of reach for the fly after 5 s. j, Median calcium signals during resting bouts on and off the treadmill (N = 6 flies, n = 68–111 bouts in total); bouts are at least 1 s in duration. Distributions, kernel density estimations; boxes, IQR and median, whiskers extend up to 1.5 × IQR.