Extended Data Fig. 2: HJs stabilize the SC central region but are dispensable for the axis-loop organization of pachytene chromosomes.
From: Holliday junction–ZMM protein feedback enables meiotic crossover assurance
a, Representative images of meiotic chromosome spreads at indicated times in SPM, immunostained for Ecm11-Gmc2 (green) and Smt3 (blue). Yen1ON expression was induced by β-oestradiol addition (or MeOH as control) at 7 h in SPM. Scale bars = 2 µm. b, c, Quantification of Ecm11-Gmc2 synapsis (b) and SC-associated Smt3 (c) from (a) (n = 50 nuclei per time point; representative of two biological replicates). d, Representative images of meiotic chromosome spreads at indicated times in SPM, immunostained for Zip1 (green) and Rec8 (magenta). Yen1ON expression was induced by β-oestradiol addition (or MeOH as control) at 7 h in SPM. Scale bars = 2 µm. e, Quantification of chromosome axis morphology based on Rec8 immunostaining from (d) (n = 50 nuclei per time point; representative of two biological replicates). f, Representative STED microscopy images of meiotic chromosome spreads at indicated times in SPM, immunostained for Zip1 (green) and Rec8 (magenta). Yen1ON expression was induced by β-oestradiol addition (or MeOH as control) at 7 h in SPM. Insets show magnified regions indicated by dashed lines. Arrowheads indicate example chromosome regions with split axes. Scale bars = 1 µm; scale bars in insets = 200 nm.
