Fig. 2: CD61 is required for MPC identity.

a, Immunofluorescence analysis of CD61 (red), YFP (grey) and SPP1 (green) expression in KPCY orthotopic tumours (n = 3 mice). Scale bar, 50 μm. b, Comparative RT–qPCR analysis of the epithelial markers Krt19, Cdh1 and Epcam and the mesenchymal markers Fn1, S100a4 and Vim in Itgb3^WT/WT KPFV and Itgb3^Δ/Δ KPFV organoids (n = 3 biological replicates). Values are normalized to Itgb3^WT/WT KPFV values. c, Comparative analysis of VIM–GFP expression between Itgb3^WT/WT KPFV and Itgb3^Δ/Δ KPFV organoids. Arrowheads indicate VIM–GFP⁺ cells. Scale bar, 50 μm. d, Images of subcutaneous tumours formed from Itgb3^WT/WT KPF and Itgb3^Δ/Δ KPF organoids. Scale bar, 0.5 cm. e, Sizes of subcutaneous tumours formed from Itgb3^WT/WT KPF and Itgb3^Δ/Δ KPF organoids (n = 4 mice). f, Immunofluorescence staining for GFP (grey), KRT19 (green) and VIM (red) of Itgb3^WT/WT KPF and Itgb3^Δ/Δ KPF subcutaneous tumours. Arrowheads indicate VIM⁺ cancer cells. Scale bar, 50 μm. g, Comparison of the percentages of different cancer cell subpopulations between subcutaneous tumours formed from Itgb3^WT/WT KPF and Itgb3^Δ/Δ KPF organoids (n = 3 mice). KRT19⁺VIM^– marks epithelial cancer cells, whereas KRT19⁺VIM⁺ marks EMT hybrid cancer cells, and KRT19^–VIM⁺ indicates mesenchymal cancer cells. Data are the mean ± s.e.m. (b,e,g). P values were calculated using two-sided t-tests.

Source Data