Extended Data Fig. 9: Strains with shorter CRISPR arrays exhibited stronger resistance to MDAΦ lysogenization upon acquisition induction.

a, Nme 8013 strain derivatives, including those with varying-sized arrays and natural escaper e5, were infected with MDAΦ in the presence or absence of IPTG to induce Cas1-Cas2 OE. Plotted are ratios of CFU/mL comparing conditions with versus without IPTG-induced acquisition, with white bars representing transductants and grey bars representing total viable cells. Data are mean ± s.d., n = 3. NS, not significant (P ≥ 0.05), * 0.005 ≤ P < 0.05, ** P < 0.005; P values calculated by two-tailed Welch’s t-tests. b, Fold changes in transduction frequencies comparing IPTG-induced acquisition versus uninduced conditions from the same experiment in panel a. Transduction frequency is the percentage of recipient cells successfully transduced. Data are mean ± s.d., n = 3. NS, not significant (P ≥ 0.05), * 0.005 ≤ P < 0.05, ** P < 0.005; P values calculated by two-tailed Welch’s t-tests.