Extended Data Fig. 5: KCTD10 does not directly regulate TOP2 activity.
From: KCTD10 is a sensor for co-directional transcription–replication conflicts
a, western blot from whole cell lysates (WCL) and chromatin fractions from KCTD10-deficient or control U2OS cells treated with ICRF193 (1 μM) or DMSO for the indicated. b, mitotic progression assay in KCTD10-deficient or control U2OS cells. Left, experimental schema to test mitotic progression. Right, quantification of cells at the indicated cell cycle stages, 2 h after release from G2/M block. Data are presented as mean values ± SD (n = 3 biologically independent experiments). c, G2/M checkpoint assay in KCTD10-deficient or control U2OS cells. Left, representative images of interphase (pHH3-) and mitotic (pHH3 + ) cells. Top right, experimental schema for G2/M checkpoint assay. Bottom right, quantification of pHH3+ cells. Data are presented as mean values ± SD (n = 3 biologically independent experiments). Scalebars = 10 μm. p-values reported from a two-way ANOVA with Dunnett’s multiple comparisons test (b) and a one-way ANOVA with Bonferroni’s multiple comparisons test (c).
