Fig. 2: KCTD10 responds to co-directional TRCs.
From: KCTD10 is a sensor for co-directional transcription–replication conflicts
a, Quantification of POLR2A–PCNA PLA foci from KCTD10-deficient (sh2 and sh3) or control HEK293T cells expressing the indicated reporters8 as shown in Extended Data Fig. 2i (top), with schemas showing the orientation of replication and transcription (bottom). Cells were treated with 1 mg ml−1 doxycycline (Dox) for 24 h to induce TRCs. Data are mean ± s.d. (n = 3 biologically independent experiments). Data are normalized to the number of foci in control cells treated with doxycycline. CD, co-directional TRCs; HO, head-on TRCs. b, Quantification of co-directional and head-on TRCs among KCTD10-associated peaks from CUT&RUN sequencing in HeLa cells expressing Flag-tagged KCTD10 treated with DMSO control or ICRF193 (100 nM for 4 h). c, Western blot analysis of KCTD10-deficient or control U2OS cells. d, Colony-formation assays in KCTD10-deficient or control U2OS cells treated with ATMi (KU55933, top) or CHK2i (BML277, bottom). Data are mean ± s.d. (n = 3 biologically independent experiments). P values were calculated with one-way ANOVA with Bonferroni’s multiple comparisons test.
