Extended Data Fig. 5: Validation of astrocyte Fos tagging approach: Temporal window of astrocyte Fos tagging with fear recall.
From: The astrocytic ensemble acts as a multiday trace to stabilize memory
a, Schematic illustrating AAV-PHP.eB vector i.v. injection into Fos-iCreERT2 (TRAP2) mice for expressing green fluorescence reporter mNeonGreen (mNG) in Fos+ astrocytes. b, Timeline of fear recall experiment to determine effective time window of astrocyte Fos tagging. c, Example images of Fos-tagged (mNG-positive) astrocytes in the lateral and basolateral amygdala (LA/B) of TRAP2 mice that underwent the fear recall experiment (representative of 17–48 slices from 3–8 mice/group). d, NS: n = 24 slices from 4 mice, −12 h: n = 30 slices from 5 mice, −6 h: n = 42 slices from 7 mice, −3 h: n = 23 slides from 4 mice, 0 h: n = 48 slices from 8 mice, 3 h: n = 30 slices from 5 mice, 6 h: n = 18 slices from 3 mice, 12 h: n = 17 slices from 3 mice. Two-way ANOVA, Šídák’s multiple comparisons test. Data are mean ± SEM. ****p < 0.0001. Sample sizes and replicates in Supplementary Table 1.
