Extended Data Fig. 7: Growth factors known to induce neuronal c-Fos did not induce astrocyte c-Fos.
From: The astrocytic ensemble acts as a multiday trace to stabilize memory
a, Schematics illustrating the design of pharmacological screening. b, Representative images from 9 biological replicates showing high purity of primary astrocyte culture. The immunoreactivities of Iba1, a microglia marker, Tuj1, a neuronal marker and Olig2, an oligodendrocyte marker, were not evident, and most of the DAPI signal overlapped with GFAP-positive primary astrocytes. c, Graph showing that c-Fos intensity in GFAP-positive astrocytes was increased with noradrenaline (NA), but not with growth factors that are known to induce c-Fos expression in primary neurons. TTX (1 µM) was used throughout the culture experiments to eliminate the potential effect of neuronal firing. Veh: n = 41 cells, NA: n = 45 cells, BDNF: n = 29 cells, NT-3: n = 33 cells, NT-4: n = 41 cells, NGFβ: n = 35 cells, EGF: n = 36 cells, PDGF-AA: n = 39 cells, PDGF-BB: n = 35 cells, CNTF: n = 40 cells, IGF: n = 46 cells. One-way ANOVA, Dunnett’s test (vs Veh). Data are mean ± SEM. ****p < 0.0001; ns, not significantly different. Sample sizes and replicates in Supplementary Table 1.
