Extended Data Fig. 11: scRNA-seq of amygdalar astrocytes.
From: The astrocytic ensemble acts as a multiday trace to stabilize memory
a, Representative gating strategy of FACS-sorting of ACSA2+ astrocytes from the amygdala for scRNA-seq. b, Violin plot showing relative expression levels of cell type marker genes in scRNA-seq. Aldoc, an astrocyte marker, was the only gene showing high expression. c, tSNE map showing astrocyte genes Aldoc, Atp1a2 (coding astrocyte cell surface antigen-2, ACSA2 with which we aimed to FACS-sort astrocytes from the amygdala tissue), and Slc1a3 with which we identified high expression in astrocytes across amygdalar astrocytes in our RNAscope experiments. d, Left: heatmap showing the top 20 genes that were upregulated in each astrocyte cluster relative to the others. Right: Example cluster marker genes identified were plotted onto the tSNE map. See also Supplementary Table 3. e, Left: tSNE plot of astrocyte clusters to illustrate that the 4 clusters are found in every single mouse examined. Right: Bar graph showing the proportion of each astrocyte cluster in the four experimental groups. n = 16 mice in b-d, 4 mice/group in e.
