Extended Data Fig. 12: Astrocytic Igfbp2 stabilizes memory during reconsolidation.
From: The astrocytic ensemble acts as a multiday trace to stabilize memory
a, Top 10 differentially expressed gene candidates, ranked by p value comparing Adrb1-positive vs. -negative astrocytes. Heatmap shows decreased (blue) or increased (red) expression. b, Violin plot showing Igfbp2 enrichment in Adrb1-positive astrocytes. n = 293 Adrb1–, 87 Adrb1+ astrocytes. Mann–Whitney test. c, RNAscope images (representative of 588 cells from 6 mice) of Adrb1 and Igfbp2 in Slc1a3-expressing astrocytes in LA/B from FR. Arrow: astrocyte with high Adrb1 and Igfbp2. d, Correlation of Igfbp2 and Adrb1 levels. Igfbp2-high astrocytes (z > 0.5) = 24% of astrocytes. Rationale in Methods. e, Adrb1 expression enriched in Igfbp2-high astrocytes (n = 451 low, 137 high cells). Igfbp2 expression enriched in Adrb1-high astrocytes (n = 426 low, 162 high cells). Mann–Whitney test. Lines, median. f, Schematic of in vivo Igfbp2 perturbation. Mice implanted with bilateral cannulae into LA/B for Igfbp2-neutralizing antibody or control IgG infusion immediately after FR. g, Scatter graph: IgG infusion (n = 9 mice) showed no change in freezing between 1st and 2nd FR, whereas Igfbp2 antibody infusion (n = 12 mice) reduced freezing in 2nd FR, indicating destabilized memory. Wilcoxon signed-rank one-tail test. h, Freezing behaviour during 5-day Igfbp2 antibody infusions into LA/B within reconsolidation windows. n = 9 mice IgG, 12 mice Igfbp2 antibody. Two-way ANOVA with Šídák’s test. i, Cannula placement in LA/B. Sites mapped on a standard atlas (adapted from Paxinos, G. & Franklin, K.B.J. The Mouse Brain in Stereotaxic Coordinates, 2nd ed. Academic Press, 2001). Each circle indicates cannula termination, coloured by group. n = 9 mice IgG, 12 mice Igfbp2 antibody. j, Representative LA/B image showing Alexa-labelled antibody infusion (representative of 36 sections from 21 mice). *p < 0.05, **p < 0.01, ****p < 0.0001; ns, not significant. Data are mean and SEM (error bars smaller than symbols in some cases). Full details in Supplementary Table 1.
