Extended Data Fig. 1: Validation of astrocyte Fos tagging approach: brain-wide astrocyte selectivity.
From: The astrocytic ensemble acts as a multiday trace to stabilize memory
a, Schematic illustrating an AAV PHP.eB vector intravenous (i.v.) injection into retro orbital sinus of adult mice for expressing red fluorescence reporter mScarlet-I in astrocytes in whole brain. b, Representative images showing that whole-brain expression of mScarlet-I was observed in AAV injected mice, but not in saline injected mice. AAV PHP.eB-mediated mScarlet-I delivery resulted in expression within S100β/GFAP positive astrocytes. Subpanel b’ shows mScarlet-I expression in the orbitofrontal cortex (ORB), the prelimbic cortex (PL), the anterior insular cortex (AIC), the dorsomedial striatum (DMS), the nucleus accumbens (ACB), the mediodorsal thalamus (MD), and the lateral and basal amygdala (LA/B). Representative image from 3 mice. c, Bar plots showing that 97.3%–99.5% of astrocytes were positive for mScarlet-I, whose signals were enhanced with anti-RFP antibody, in the indicated 7 brain regions (left) and that those regions had few mScarlet-I-positive neurons (right). n = 3 mice. Data are mean ± SEM (error bars smaller than symbols in some cases). Sample sizes and replicates in Supplementary Table 1.
