Extended Data Fig. 2: Validation of astrocyte Fos tagging approach: Fos upregulation sensitivity.
From: The astrocytic ensemble acts as a multiday trace to stabilize memory
a, Schematic illustrating AAV-PHP.eB vector i.v. injection into Fos-iCreERT2 (TRAP2) mice for expressing green fluorescence reporter mNeonGreen (mNG) in Fos+ astrocytes. AAV2/5-GfaABC1D-hM3Dq-mCherry was stereotaxically delivered into the orbitofrontal cortex (ORB) to induce Fos in astrocytes by 1 mg/kg CNO i.p. injection and tag those Fos+ astrocytes with mNG by 50 mg/kg 4-hydroxytamoxifen (4-OHT) i.p. injection. b, hM3Dq activation with CNO administration increased Fos expression in ORB astrocytes. hM3Dq-, CNO+: n = 12 slices, hM3Dq+, CNO-: n = 12 slices, hM3Dq+, CNO+: n = 12 slices, hM3Dq-, 3 mice/group. ****p < 0.0001. One-way ANOVA, Tukey’s test. Data are mean ± SEM. Sample sizes and replicates in Supplementary Table 1.
