Extended Data Fig. 3: Validation of astrocyte Fos tagging approach: Temporal window of astrocyte Fos tagging with visual stimulation.
From: The astrocytic ensemble acts as a multiday trace to stabilize memory
a, Schematic illustrating AAV-PHP.eB vector i.v. injection into Fos-iCreERT2 (TRAP2) mice for expressing green fluorescence reporter mNeonGreen (mNG) in Fos+ astrocytes. b, Timeline of visual stimulation experiment to determine effective time window of astrocyte Fos tagging. c, Example images of Fos-tagged (mNG-positive) astrocytes in the primary visual cortex (VISp) of TRAP2 mice that underwent the visual stimulation experiment (representative of 9–12 slices from 3-4 mice/group). d, Quantification of mNG astrocyte density in TRAP2 mice VISp and primary somatosensory cortex (SSp): NS: n = 12 slices from 4 mice, −24 h: n = 12 slices from 4 mice, −12 h: n = 12 slices from 3 mice, −6 h: n = 12 slices from 4 mice, −3 h: n = 12 slides from 4 mice, 0 h: n = 12 slices from 4 mice, 3 h: n = 9 slices from 3 mice, 6 h: n = 9 slices from 3 mice, 12 h: n = 9 slices from 3 mice, 24 h: n = 9 slices from 3 mice. Two-way ANOVA, Šídák’s multiple comparisons test. Data are mean ± SEM. *p < 0.05, ***p < 0.001, ****p < 0.0001. Sample sizes and replicates in Supplementary Table 1.
