Extended Data Fig. 1: Histopathological evaluation of the nonhuman primate (NHP) spinal cord at three months following a single IT dose of 60 mg C16-siRNA.
From: Expanding RNAi therapeutics to extrahepatic tissues with lipophilic conjugates
Representative H&E (a), LFB (b) and IBA-1 (c) staining of NHP thoracic spinal cord. Microscopic findings in the spinal cord were limited to procedure-related changes (that is, IT bolus administration in lumbar region). These consisted of minimal to mild degeneration of nerve fibers with dilated axonal sheaths and few digestion chambers containing gitter cells (arrowheads) that were localized to the white matter of spinal cord (cervical, thoracic, and lumbar segments). Limited to procedure-affected areas, there were minimal decreases in LFB staining intensity (arrows) and a minimal increase in the number of IBA-1 (brown chromogen) positive microglia (open arrows). Three animals were analyzed per group with similar results. GM = grey matter; WM = white matter.
