Extended Data Fig. 8: UAV acquisition of HSI data to detect bacteriochlorophyll a from 24 m.
R. gelatinosus WT, YF6 (constitutive bacteriochlorophyll a), and AJ1 (repressed bacteriochlorophyll a) cultures were grown to OD600 = 4.0 (Methods). Cultures were diluted using growth media or concentrated using centrifugation and resuspension in growth media to achieve different OD600 of culture cell density added to soil. 300 mL of each culture was then sprayed on 1.5 kg locally-sourced sandy soil inside each 35 ×30 cm plastic container. Containers were then placed in a field in a closed area near Herzliya, Israel. Imaging was performed with a VNIR-SWIR (400-2500 nm) UAV hyperspectral camera (Co-Aligned HP Hyperspectral Imaging UAV and camera, Headwall) from 24 m. Using SpectralView III, reflectance correction was performed based on the calibration tarp and geo-orthorectification was performed to correct for image distortions from the flight movement. a. Picture showing a close-up of the UAV imaging system and the containers in the background. b. RGB image from the HSI data. c. Classified image from the HSI. d. Locations of different R. gelatinosus strains and their concentrations used in the experiment. The AJ1 strain was used at OD600 = 4.0. The six concentrations in the center box correspond to the wild-type strain; the two concentrations flanking the box (left and right) correspond to the YF6 strain. e. Uncropped RGB image. f. Uncropped classified image. The image in panels e and f covers 0.2 acres (800 m2).
