Extended Data Fig. 10: Measurement of the 3OH-C14-HSL sensor response function from 90 m using bacteriochlorophyll a.

These data correspond to Fig. 4j using R. gelatinosus YF10. The top image shows the whole area shown in Fig. 4h. The bottom images are enlarged images of the area marked by the white rectangles in the top images. The black rectangle in the bottom images marks the area in the image where R. gelatinosus YF10 was added onto 1 kg of sand. The sand covered a 1 cm deep filter disc (60 mm) treated with 800 µL of 19 mM 3OH-C14-HSL (final concentration of 50 µM/mL culture). a. RGB image calculated from HSI data. b. 864 nm image calculated from HSI data. c. Data used to calculate the ROC curve for 864 nm (Fig. 4j). Pixel absorption at 864 nm with different thresholds used to label pixels as containing HSR are shown. Values in the heatmap (panel b) greater than the threshold were labeled as containing HSR (red) and values equal to or less than the threshold were labeled as not containing HSR (gray). Images represent four of 470,044 threshold values evaluated to compute the ROC curve (Methods). d. Data used to calculate the classified ROC curve (Fig. 4j). Classified HSI images are shown at different threshold values taken along the ROC curve. Values in the classified image (Fig. 4h) greater than the threshold were labeled as containing HSR (red) and values equal to or less than the threshold were labeled as not containing HSR (gray). Images represent four of 1,015,973 threshold values evaluated to compute the ROC curve (Methods).