Extended Data Fig. 4: pC-HSL response functions measured by HSR and a fluorescent reporter.
a. Schematics of genetic sensors connected to the different reporters. Plasmids are detailed in Supplementary Fig. 6 and genetic parts in Supplementary Table 1. b. The response functions as measured with the two reporters. In both cases, cells were grown with different concentrations of inducers (Methods). The pC-HSL sensor connected to YFP (E. coli pYC303) was imaged using a fluorimeter and background fluorescence (fluorescence of the pellets at inducer concentration = 0) was subtracted. The lines are fits to Eq. 6 (HSR: K = 2.2 nM, n = 1.1, YFP: K = 15.4 nM, n = 1.2). The data points are three replicates.
