Extended Data Fig. 5: CRISPR screening of targets affecting mutant cell fitness.
From: Organismal metabolism regulates the expansion of oncogenic PIK3CA mutant clones in normal esophagus
a-b, CRISPR screening in uninduced (Pik3cawt/wt, WT) cells in minimal medium (CTL). a, Correlation between normalized read counts for the sequenced library and the average normalized read counts at Time 0. Orange line, linear regression between samples with the Pearson’s coefficient and two-tailed p-value of the correlation. b, Violin plots of distribution of average log2 fold change between Time 3 and Time 0 for each gRNA. n = 2 biological replicates, 10 gRNA per gene. c, Heatmaps of average log2 (fold change per gene of gRNA abundance after 3 weeks versus Time 0. Genes are grouped by pathway. Heatmaps show the enrichment of gRNAs targeting each gene at 3 weeks over 0 weeks in either minimal medium (CTL) or minimal medium supplemented with 5 μg/ml Insulin (INS), and either uninduced (WT) or induced (Pik3ca*/wt) cells from Rosa26Cas9/wt Pik3caH1047R/wt mice. Each column is a biological replicate. n = 2-3 biological replicates, 10 gRNA per gene.
