Extended Data Fig. 10: Higher density of Pik3ca missense mutations in overweight humans.
From: Organismal metabolism regulates the expansion of oncogenic PIK3CA mutant clones in normal esophagus
a, Age distribution of all human donors, including donors from Fig. 1. Lines are mean±s.e.m. Two-tailed unpaired t-test. b-d, Human donors were classified into OW-OB (n = 10, BMI ≥ 25 kg m2) and non-OW (n = 8, BMI < 25 kg m2). PIK3CA missense mutations (MM) were classified as Path/GoF and Unkn/NE (see Methods) and analyzed separately in OW-OB and Non-OW groups. Each dot represents a donor. b, Number of PIK3CA missense mutant clones detected/cm2 in the specified groups. Bars are mean±s.e.m. Two-tailed unpaired t-test. c, Summed variant allele frequency (VAF) of all Path/GoF or Unkn/NE PIK3CA missense mutations per cm2 for each donor. Lines are mean±s.e.m. Two-tailed Mann-Whitney test. d, Number of clones/cm2 carrying Path/GoF PIK3CA missense mutations in each p110α domain. Bars are mean±s.e.m. Two-tailed Mann-Whitney test.
