Extended Data Fig. 6: Additional cytokine secretion analysis.

a, Heatmap as shown in Fig. 3a with additional cytokine and chemokine values upon IFN-γ stimulation. Z-score scaling for each cytokine or chemokine shown for two individual donors. b, TNF expression in UMAP projection. c, Gene expression of CCL3, CCL4, and CXCL8 assessed by qPCR of perturbed hMDMs 18 h after IFN-γ priming with and without addition of TNFRII-Fc in culture medium; each symbol represents an individual donor (n = 5). Summary data of 3 individual experiments are shown as mean, with P values determined by one-way ANOVA with Sidak’s multiple comparisons test. d, Heatmap showing P03 genes in non-stimulated, IFN-γ primed and IFN-γ and TNFα co-stimulated cells in hMDMs, each column represents an individual donor (n = 3).