Fig. 5: Visualization of desmosomes at keratinocyte cell junctions and DSP knockdown.
From: Desmosome mutations impact the tumor microenvironment to promote melanoma proliferation
a, Representative image from keratinocyte monocultures expressing stable nontargeting control (NTC) shRNA, depicting the staining for keratin (KRT14, cyan), DSP (purple) and DSG2 (yellow). Cell nuclei were stained with DAPI (gray). Each stain is shown separately and in a single image overlay. The images show the robust expression and expected location of desmosome components (DSP, DSG2). b, Same as in a but for keratinocyte monocultures expressing stable DSP shRNA, illustrating the effective knockdown of the desmosome proteins DSP and DSG2. Each experiment was repeated independently three times with similar results, with individual replicate values summarized in Extended Data Fig. 5.
