Extended Data Fig. 2: Structural features of the TRIM7^PRY-SPRY-2C peptide complex.

a, The overall structures of TRIM7^PRY-SPRY-2C peptide complex solved in different space groups (top). Bottom, 2Fo-Fc electron density map of the 2C fragments contoured at 1.0 σ. b, Structural comparison of the three TRIM7^PRY-SPRY-2C peptide structures and a previously published substrate-free structure (PDB ID: 6UMA). c, ITC measurement of TRIM7^PRY-SPRY-2C^ΔN association in a high salt buffer containing 500 mM NaCl. d, Conservation scores across different species were mapped to the TRIM7 structure. Multiple sequence alignment of human TRIM7 and orthologs in other mammals, birds and amphibians were performed with Clustal Omega. The multiple sequence alignment and the resolved structure of TRIM7^PRY-SPRY were used as inputs for conservation analysis using the ConSurf web server (https://consurf.tau.ac.il/).