Extended Data Fig. 5: Structure of ciprofloxacin (CIP) in complex with the 70S ribosome.
From: Macrolones target bacterial ribosomes and DNA gyrase and can evade resistance mechanisms
(a) 2Fo-Fc Fourier electron density map of ciprofloxacin (CIP, greencyan) in complex with the T. thermophilus 70S ribosome (blue mesh). The refined model of ribosome-bound CIP is displayed in its respective electron density map after the refinement contoured at 1.0σ. Carbon atoms are colored greencyan; nitrogen atoms are blue; oxygen atoms are red; fluorine atom is dark green. (b, c) Close-up views of CIP bound in the NPET of the 70S ribosome, highlighting its stacking (red arrows) interactions with the nucleotides of the 23S rRNA. (d) Superposition of the structures of ribosome-bound CIP and MCX-128 (yellow). The structures were aligned based on domain V of the 23S rRNA.
