Extended Data Fig. 6: Macrolones inhibit DNA gyrase activity in vitro.
From: Macrolones target bacterial ribosomes and DNA gyrase and can evade resistance mechanisms
(a) Chemical structures of the macrolones used in the assay. (b) Effect of macrolones on the activity of DNA gyrase. Supercoiled or relaxed plasmid DNA bands are marked on the left. CIP and ERY were used as positive and negative controls, respectively. The sample labeled as ‘NONE’ contained no antibiotics. A control sample where gyrase was not added is marked as ‘-’.The experiment was repeated independently and produced similar results.
