Extended Data Fig. 2: Effects of macrolones on in vitro translation.
From: Macrolones target bacterial ribosomes and DNA gyrase and can evade resistance mechanisms
Mapping the sites of macrolone-mediated ribosome arrests (blue arrows) at the early codons of the model ORF derived from the E. coli yrbA gene. The classic macrolide ERY is included for comparison. Due to the presence of the Thr-RS inhibitor borrelidin, the ribosomes that did not stall at the early codons are eventually trapped at the Gln12 codon when Thr13 needs to be incorporated into the growing protein (grey arrow). The AUG start codon is marked with a black arrow. The sample labeled as ‘NONE’ contained only borrelidin but no ERY or macrolones. Amino acid and nucleotide sequences of yrbA gene are shown on the left. Sequencing lanes are marked as C, U, A, G. This experiment was repeated independently twice and produced similar results.
