Extended Data Fig. 10: CTB-interacting glycoproteins.
From: Fucosylation of glycoproteins and glycolipids: opposing roles in cholera intoxication
(A) Top 50 proteins identified from CTB-biotin pulldown from B3GALT5 + B3GNT5-dKO versus B3GALT5 + SLC35C1-dKO cell lysates. Fold-change is the abundance in the B3GALT5 +  B3GNT5-dKO pulldown as compared to the B3GALT5 + SLC35C1-dKO pulldown. Unadjusted P-values are derived from two-tailed student’s t-test. (B) Gene ontology (GO) analysis using Fisher’s exact test with Bonferroni correction to identify pathways enriched in CTB-biotin pulldown from B3GALT5 + B3GNT5-dKO cell lysates as compared to B3GALT5 + SLC35C1-dKO cell lysates. Top 25 pathways are shown. (C) Two hits (FLOT1 and MUC1, boldface font in panel A) were selected for confirmation. Total lysates and material from CTB-biotin pulldowns were analyzed by immunoblot using antibodies against FLOT1 and MUC1.
