Extended Data Fig. 1: Comparison of nutrient sensing pathways when subjected to glucose concentration changes or Opto-OGT activation.
From: Spatiotemporal control of subcellular O-GlcNAc signaling using Opto-OGT
a, Representative Western blots of key nutrient sensing pathways when HEK293T cells are subjected to glucose concentration changes from 0 mM to 25 mM. Immunoblots shown are representative of two independent biological repeats. b, Representative Western blots of HEK293T cells transfected with OGT-mCherry-CRY2 undergoing 15 minutes of blue light. Key downstream signaling pathways are probed as labelled. Immunoblots shown are representative of two independent biological repeats. c, Representative Western blots of phosphor-ULK1 when HEK293T cells transfected with OGT-mCherry-CRY2 underwent 15 minutes of blue light. Immunoblots shown are representative of two independent biological repeats.
