Extended Data Fig. 5: Quantification of potential cross-feeding substances.
From: De novo biosynthesis of plant lignans by synthetic yeast consortia
Schematic representation of methionine metabolism (a) and adenosine metabolism (b) in S. cerevisiae. The blue and yellow highlight the metabolites that are able to be found in the co-culture medium, which may mediate cross-feeding between two yeast auxotrophs (ade2Δ and met15Δ) from a yeast consortium with obligate mutualism. The color lines represent metabolic flux generated by the potential cross-feeding metabolites intending to compensate for the synthesis of all deficient metabolites of yeast auxotrophs. Extracellular concentrations of methionine-related (c) and adenosine-related (d) metabolites exported by the yeast consortia at different time points. All the data are presented as the means of n=5 independent biological samples, and the error bars show the SDs. Abbreviations: MET15: encoding O-acetyl-homoserine amino-carboxy-propyl-transferase; ADE2: encoding phosphoribosyl-aminoimidazole carboxylase; HCY, homocysteine; SAM, S-adenosyl-L-methionine; SAH, S-adenosyl-L-homocysteine; PRPP, 5-phosphoribosyl-1-pyrophosphate; AIR, 5’-phosphoribosylaminoimidazole; IMP, inosine 5’-monophosphate; AMP, adenosine 5'-monophosphate; ADP, adenosine 5'-diphosphate; ATP, adenosine 5'-triphosphate; XMP, xanthosine 5’-monophosphate; GMP, guanosine 5'-monophosphate; GDP, guanosine 5'-diphosphate; GTP, guanosine 5'-triphosphate. Gene names are italicized, and corresponding enzymatic activities of the biosynthesis pathway are indicated.
