Extended Data Fig. 6: Laccase screening for the synthesis of pinoresinol through dimerization of CoAl.
From: De novo biosynthesis of plant lignans by synthetic yeast consortia
a, Laccase (Lac) screening for pinoresinol production from CoAl. b, Peroxidase (Pex) screening for the synthesis of pinoresinol through dimerization of CoAl. The high redox potential Lac (Cu2+) obtains electrons from the coniferyl alcohol to form the reduced Lac (Cu+), which then transfers electrons to oxygen to form water. In the process, the coniferyl alcohol forms single electron free radical intermediates, and the coupling reaction produces pinoresinol. c, Electron-transfer mechanism of the redox reaction catalyzed by Lac. The relatively high redox potential of Lac (Cu2+) is needed to obtain one electron from CoAl and catalyze the dimerization. All the strains were grown at 30 °C in 20 ml of Delft-D medium and 100 mg l-1 ferulic acid. All the data are presented as the means of n=3 independent biological samples, and the error bars show the SDs. d, Color reaction of Lac coupled with ABTS after the signal peptide was removed. The depth of blue reflects the strength of the radical coupling reaction. Scale bar: 1cm. ABTS: 2,2’-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt). See Fig. 1 legends for abbreviations of enzymes and metabolites.
