Extended Data Fig. 7: Effect of excess FA feeding on pinoresinol synthesis.
From: De novo biosynthesis of plant lignans by synthetic yeast consortia

a, Schematic showing the specific method by which two doses of 50 mg l−1 FA were fed every 24 hours and RB69tr fermentation samples were collected at different time points. b, Titers of CoAl and pinoresinol in the samples at different time points. Samples were taken every three hours after FA feeding. See Fig. 1 legends for abbreviations of enzymes and metabolites. c, Substrate promiscuity of TsLac3tr. The engineered strain RB86 expressed only TsLAC3tr in the parent strain CEN. PK113-11C to test the broad substrate spectrum. All the feeding substrates (100 mg l−1 p-coumaric acid, caffeic acid, ferulic acid, and coniferyl alcohol) were consumed, indicating the promiscuity of the TsLac3tr function and the broad substrate spectrum. All the strains were subsequently grown at 30 °C in 20 ml of Delft-D medium. All the data are presented as the means of n=3 independent biological samples, and the error bars show the SDs.